Abstract
The inhibitory glycine receptor (GlyR) is a ligand-gated ion channel which mediates post-synaptic inhibition in spinal cord and other regions of the vertebrate central nervous system. Previous biochemical and molecular cloning studies have indicated heterogeneity of GlyRs during development. Here, the distribution of GlyR subunit transcripts in rat brain and spinal cord was investigated by in situ hybridization using sequence-specific oligonucleotide probes. In adult animals, GlyR α1 subunit mRNA was abundant in spinal cord, but was also seen in a few brain areas, e.g. superior and inferior colliculi, whereas α2 transcripts were found in several brain regions including layer VI of the cerebral cortex and hippocampus. GlyR α3 subunit mRNA was expressed at low levels in cerebellum, olfactory bulb and hippocampus, while high amounts of β subunit transcripts were widely distributed throughout spinal cord and brain. During development, α2 mRNA accumulated already prenatally and decreased after birth, whereas α1 and α3 subunit transcripts appeared only in postnatal brain structures. Hybridization signals of β subunit mRNA were seen already at early embryonic stages and continuously increased to high levels in adult rats. These data reveal unexpected differences in the regional and developmental expression of GlyR subunit mRNAs and point to novel functions of GlyR proteins in the mammalian central nervous system.
| Original language | English |
|---|---|
| Pages (from-to) | 2401-2409 |
| Number of pages | 9 |
| Journal | EMBO Journal |
| Volume | 10 |
| Issue number | 9 |
| Publication status | Published - 1991 |
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Keywords
- Glycine receptor
- Heterogeneity
- In situ hybridization
- mRNA distribution
- Rat brain
ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain. / Malosio, Maria Luisa; Marquèze-Pouey, Béatrice; Kuhse, Jochen; Betz, Heinrich.
In: EMBO Journal, Vol. 10, No. 9, 1991, p. 2401-2409.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain
AU - Malosio, Maria Luisa
AU - Marquèze-Pouey, Béatrice
AU - Kuhse, Jochen
AU - Betz, Heinrich
PY - 1991
Y1 - 1991
N2 - The inhibitory glycine receptor (GlyR) is a ligand-gated ion channel which mediates post-synaptic inhibition in spinal cord and other regions of the vertebrate central nervous system. Previous biochemical and molecular cloning studies have indicated heterogeneity of GlyRs during development. Here, the distribution of GlyR subunit transcripts in rat brain and spinal cord was investigated by in situ hybridization using sequence-specific oligonucleotide probes. In adult animals, GlyR α1 subunit mRNA was abundant in spinal cord, but was also seen in a few brain areas, e.g. superior and inferior colliculi, whereas α2 transcripts were found in several brain regions including layer VI of the cerebral cortex and hippocampus. GlyR α3 subunit mRNA was expressed at low levels in cerebellum, olfactory bulb and hippocampus, while high amounts of β subunit transcripts were widely distributed throughout spinal cord and brain. During development, α2 mRNA accumulated already prenatally and decreased after birth, whereas α1 and α3 subunit transcripts appeared only in postnatal brain structures. Hybridization signals of β subunit mRNA were seen already at early embryonic stages and continuously increased to high levels in adult rats. These data reveal unexpected differences in the regional and developmental expression of GlyR subunit mRNAs and point to novel functions of GlyR proteins in the mammalian central nervous system.
AB - The inhibitory glycine receptor (GlyR) is a ligand-gated ion channel which mediates post-synaptic inhibition in spinal cord and other regions of the vertebrate central nervous system. Previous biochemical and molecular cloning studies have indicated heterogeneity of GlyRs during development. Here, the distribution of GlyR subunit transcripts in rat brain and spinal cord was investigated by in situ hybridization using sequence-specific oligonucleotide probes. In adult animals, GlyR α1 subunit mRNA was abundant in spinal cord, but was also seen in a few brain areas, e.g. superior and inferior colliculi, whereas α2 transcripts were found in several brain regions including layer VI of the cerebral cortex and hippocampus. GlyR α3 subunit mRNA was expressed at low levels in cerebellum, olfactory bulb and hippocampus, while high amounts of β subunit transcripts were widely distributed throughout spinal cord and brain. During development, α2 mRNA accumulated already prenatally and decreased after birth, whereas α1 and α3 subunit transcripts appeared only in postnatal brain structures. Hybridization signals of β subunit mRNA were seen already at early embryonic stages and continuously increased to high levels in adult rats. These data reveal unexpected differences in the regional and developmental expression of GlyR subunit mRNAs and point to novel functions of GlyR proteins in the mammalian central nervous system.
KW - Glycine receptor
KW - Heterogeneity
KW - In situ hybridization
KW - mRNA distribution
KW - Rat brain
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UR - http://www.scopus.com/inward/citedby.url?scp=0025734108&partnerID=8YFLogxK
M3 - Article
C2 - 1651228
AN - SCOPUS:0025734108
VL - 10
SP - 2401
EP - 2409
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 9
ER -