Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain

Maria Luisa Malosio, Béatrice Marquèze-Pouey, Jochen Kuhse, Heinrich Betz

Research output: Contribution to journalArticle

421 Citations (Scopus)

Abstract

The inhibitory glycine receptor (GlyR) is a ligand-gated ion channel which mediates post-synaptic inhibition in spinal cord and other regions of the vertebrate central nervous system. Previous biochemical and molecular cloning studies have indicated heterogeneity of GlyRs during development. Here, the distribution of GlyR subunit transcripts in rat brain and spinal cord was investigated by in situ hybridization using sequence-specific oligonucleotide probes. In adult animals, GlyR α1 subunit mRNA was abundant in spinal cord, but was also seen in a few brain areas, e.g. superior and inferior colliculi, whereas α2 transcripts were found in several brain regions including layer VI of the cerebral cortex and hippocampus. GlyR α3 subunit mRNA was expressed at low levels in cerebellum, olfactory bulb and hippocampus, while high amounts of β subunit transcripts were widely distributed throughout spinal cord and brain. During development, α2 mRNA accumulated already prenatally and decreased after birth, whereas α1 and α3 subunit transcripts appeared only in postnatal brain structures. Hybridization signals of β subunit mRNA were seen already at early embryonic stages and continuously increased to high levels in adult rats. These data reveal unexpected differences in the regional and developmental expression of GlyR subunit mRNAs and point to novel functions of GlyR proteins in the mammalian central nervous system.

Original languageEnglish
Pages (from-to)2401-2409
Number of pages9
JournalEMBO Journal
Volume10
Issue number9
Publication statusPublished - 1991

Fingerprint

Glycine Receptors
Rats
Brain
Messenger RNA
Spinal Cord
Neurology
Hippocampus
Central Nervous System
Ligand-Gated Ion Channels
Inferior Colliculi
Oligonucleotide Probes
Superior Colliculi
Cloning
Olfactory Bulb
Molecular Cloning
Cerebral Cortex
Cerebellum
In Situ Hybridization
Vertebrates
Animals

Keywords

  • Glycine receptor
  • Heterogeneity
  • In situ hybridization
  • mRNA distribution
  • Rat brain

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain. / Malosio, Maria Luisa; Marquèze-Pouey, Béatrice; Kuhse, Jochen; Betz, Heinrich.

In: EMBO Journal, Vol. 10, No. 9, 1991, p. 2401-2409.

Research output: Contribution to journalArticle

Malosio, ML, Marquèze-Pouey, B, Kuhse, J & Betz, H 1991, 'Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain', EMBO Journal, vol. 10, no. 9, pp. 2401-2409.
Malosio, Maria Luisa ; Marquèze-Pouey, Béatrice ; Kuhse, Jochen ; Betz, Heinrich. / Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain. In: EMBO Journal. 1991 ; Vol. 10, No. 9. pp. 2401-2409.
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