Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells

C. De Giovanni, P. Nanni, A. Sacchi, S. Soddu, I. Manni, G. D'Orazi, S. Bulfone-Paus, T. Pohl, L. Landuzzi, G. Nicoletti, F. Frabetti, I. Rossi, P. L. Lollini

Research output: Contribution to journalArticle

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Abstract

We recently reported that rhabdomyosarcoma cell lines express and secrete interleukin 15 (IL-15), a tightly regulated cytokine with IL-2-like activity. To test whether the p53-impaired function that is frequently found in this tumour type could play a role in the IL-15 production, wild-type p53 gene was transduced in the human rhabdomyosarcoma cell line RD (which harbours a mutated p53 gene), and its effect on proliferation and expression of IL-15 was studied. Arrest of proliferation was induced by wild-type p53; increased proportions of G1 arrested cells and of apoptotic cells were observed. A marked down-modulation of IL-15 expression, at both the mRNA and protein level, was found in p53-transduced cells. Because a direct effect of IL-15 on normal muscle cells has been reported, the presence of IL-15 membrane receptors was studied by cytofluorometric analysis. Rhabdomyosarcoma cells showed IL-15 membrane receptors, which are down-modulated by wild-type p53 transfected gene. In conclusion, wild-type p53 transduction in human rhabdomyosarcoma cells induces the down-modulation of both IL-15 production and IL-15 receptor expression.

Original languageEnglish
Pages (from-to)1541-1546
Number of pages6
JournalBritish Journal of Cancer
Volume78
Issue number12
Publication statusPublished - 1998

Fingerprint

Interleukin-15 Receptors
Interleukin-15
Rhabdomyosarcoma
p53 Genes
Cell Line
Membranes
Muscle Cells
Interleukin-2
Cytokines
Messenger RNA

Keywords

  • Gene transduction
  • Human rhabdomyosarcoma
  • Interleukin 15
  • Interleukin-15 receptors
  • p53

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells. / De Giovanni, C.; Nanni, P.; Sacchi, A.; Soddu, S.; Manni, I.; D'Orazi, G.; Bulfone-Paus, S.; Pohl, T.; Landuzzi, L.; Nicoletti, G.; Frabetti, F.; Rossi, I.; Lollini, P. L.

In: British Journal of Cancer, Vol. 78, No. 12, 1998, p. 1541-1546.

Research output: Contribution to journalArticle

De Giovanni, C, Nanni, P, Sacchi, A, Soddu, S, Manni, I, D'Orazi, G, Bulfone-Paus, S, Pohl, T, Landuzzi, L, Nicoletti, G, Frabetti, F, Rossi, I & Lollini, PL 1998, 'Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells', British Journal of Cancer, vol. 78, no. 12, pp. 1541-1546.
De Giovanni, C. ; Nanni, P. ; Sacchi, A. ; Soddu, S. ; Manni, I. ; D'Orazi, G. ; Bulfone-Paus, S. ; Pohl, T. ; Landuzzi, L. ; Nicoletti, G. ; Frabetti, F. ; Rossi, I. ; Lollini, P. L. / Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells. In: British Journal of Cancer. 1998 ; Vol. 78, No. 12. pp. 1541-1546.
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