Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells

C. De Giovanni, P. Nanni, A. Sacchi, S. Soddu, I. Manni, G. D'Orazi, S. Bulfone-Paus, T. Pohl, L. Landuzzi, G. Nicoletti, F. Frabetti, I. Rossi, P. L. Lollini

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Abstract

We recently reported that rhabdomyosarcoma cell lines express and secrete interleukin 15 (IL-15), a tightly regulated cytokine with IL-2-like activity. To test whether the p53-impaired function that is frequently found in this tumour type could play a role in the IL-15 production, wild-type p53 gene was transduced in the human rhabdomyosarcoma cell line RD (which harbours a mutated p53 gene), and its effect on proliferation and expression of IL-15 was studied. Arrest of proliferation was induced by wild-type p53; increased proportions of G1 arrested cells and of apoptotic cells were observed. A marked down-modulation of IL-15 expression, at both the mRNA and protein level, was found in p53-transduced cells. Because a direct effect of IL-15 on normal muscle cells has been reported, the presence of IL-15 membrane receptors was studied by cytofluorometric analysis. Rhabdomyosarcoma cells showed IL-15 membrane receptors, which are down-modulated by wild-type p53 transfected gene. In conclusion, wild-type p53 transduction in human rhabdomyosarcoma cells induces the down-modulation of both IL-15 production and IL-15 receptor expression.

Original languageEnglish
Pages (from-to)1541-1546
Number of pages6
JournalBritish Journal of Cancer
Volume78
Issue number12
Publication statusPublished - 1998

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Keywords

  • Gene transduction
  • Human rhabdomyosarcoma
  • Interleukin 15
  • Interleukin-15 receptors
  • p53

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

De Giovanni, C., Nanni, P., Sacchi, A., Soddu, S., Manni, I., D'Orazi, G., Bulfone-Paus, S., Pohl, T., Landuzzi, L., Nicoletti, G., Frabetti, F., Rossi, I., & Lollini, P. L. (1998). Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells. British Journal of Cancer, 78(12), 1541-1546.