Wilms' Tumor Gene 1 Transcript Levels in Leukapheresis ofPeripheral Blood Hematopoietic Cells Predict Relapse Risk inPatients Autografted for Acute Myeloid Leukemia

Carlo Messina, Anna Candoni, Matteo G. Carrabba, Cristina Tresoldi, Elisa Sala, Michela Tassara, Alessandra Crippa, Jacopo Peccatori, Andrea Assanelli, Salvatore Gattillo, Laura Bellio, Renato Fanin, Fabio Ciceri, Massimo Bernardi

Research output: Contribution to journalArticlepeer-review

Abstract

Autologous hematopoietic stem cell transplantation (ASCT) is a curative option alternative to allogeneic transplantation for patients with acute myeloid leukemia (AML). Relapse after ASCT can be due to contamination with leukemic blasts of autologous peripheral blood stem cells (PBSCs) collected by leukapheresis (LK). Identification and quantification of a minimal residual disease (MRD) marker in PBSCs could be relevant in determining the relapse risk after ASCT. High levels of the WT1 gene transcript in bone marrow of AML patients after treatment completion predict disease relapse. We evaluated WT1 transcript levels in autologous PBSC from LK used for ASCT in 30 consecutive AML patients in complete remission (CR) and established a correlation with clinical outcome. At diagnosis, all patients had WT1 overexpression. All patients were in morphological and genetic CR at the time of PBSC collection and before ASCT. Real-time quantitative PCR of WT1 was performed in samples of each LK, using TaqMan technology on RNA from mononucleated cells. The median WT1 transcript level in the PBSC graft (WT1-LK) of patients who relapsed was significantly higher than of those who did not relapse after transplantation (P

Original languageEnglish
Pages (from-to)1586-1591
Number of pages6
JournalBiology of Blood and Marrow Transplantation
Volume20
Issue number10
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Acute myeloid leukemia
  • Autologous stem cell transplantation
  • Minimal residual disease
  • Peripheral blood stem cell apheresis
  • Real-time quantitative PCR
  • Wilms' tumor gene 1

ASJC Scopus subject areas

  • Transplantation
  • Hematology
  • Medicine(all)

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