Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor snf96.2 cell line

Rosalba Parenti, Venera Cardile, Adriana Carol Eleonora Graziano, Carmela Parenti, Assunta Venuti, Maria Paola Bertuccio, Debora Lo Furno, Gaetano Magro

Research output: Contribution to journalArticle

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Abstract

Wilms' tumor gene 1 (WT1) plays complex roles in tumorigenesis, acting as tumor suppressor gene or an oncogene depending on the cellular context. WT1 expression has been variably reported in both benign and malignant peripheral nerve sheath tumors (MPNSTs) by means of immunohistochemistry. The aim of the present study was to characterize its potential pathogenetic role in these relatively uncommon malignant tumors. Firstly, immunohistochemical analyses in MPNST sNF96.2 cell line showed strong WT1 staining in nuclear and perinuclear areas of neoplastic cells. Thus, we investigated the effects of silencing WT1 by RNA interference. Through Western Blot analysis and proliferation assay we found that WT1 knockdown leads to the reduction of cell growth in a time- and dosedependent manner. siWT1 inhibited proliferation of sNF96.2 cell lines likely by influencing cell cycle progression through a decrease in the protein levels of cyclin D1 and inhibition of Akt phosphorylation compared to the control cells. These results indicate that WT1 knockdown attenuates the biological behavior of MPNST cells by decreasing Akt activity, demonstrating that WT1 is involved in the development and progression of MPNSTs. Thus, WT1 is suggested to serve as a potential therapeutic target for MPNSTs.

Original languageEnglish
Article numbere114333
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 4 2014

Fingerprint

Wilms' Tumor Genes
Wilms Tumor
peripheral nerves
Neurilemmoma
Gene Silencing
Tumor Cell Line
Tumors
Genes
Cells
cell lines
neoplasms
genes
Gene Knockdown Techniques
neoplasm cells
Cyclin D1
RNA Interference
Tumor Suppressor Genes
Oncogenes
Cell Cycle
Carcinogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Parenti, R., Cardile, V., Graziano, A. C. E., Parenti, C., Venuti, A., Bertuccio, M. P., ... Magro, G. (2014). Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor snf96.2 cell line. PLoS One, 9(12), [e114333]. https://doi.org/10.1371/journal.pone.0114333

Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor snf96.2 cell line. / Parenti, Rosalba; Cardile, Venera; Graziano, Adriana Carol Eleonora; Parenti, Carmela; Venuti, Assunta; Bertuccio, Maria Paola; Lo Furno, Debora; Magro, Gaetano.

In: PLoS One, Vol. 9, No. 12, e114333, 04.12.2014.

Research output: Contribution to journalArticle

Parenti, R, Cardile, V, Graziano, ACE, Parenti, C, Venuti, A, Bertuccio, MP, Lo Furno, D & Magro, G 2014, 'Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor snf96.2 cell line', PLoS One, vol. 9, no. 12, e114333. https://doi.org/10.1371/journal.pone.0114333
Parenti, Rosalba ; Cardile, Venera ; Graziano, Adriana Carol Eleonora ; Parenti, Carmela ; Venuti, Assunta ; Bertuccio, Maria Paola ; Lo Furno, Debora ; Magro, Gaetano. / Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor snf96.2 cell line. In: PLoS One. 2014 ; Vol. 9, No. 12.
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