Abstract
Very little is known about Wilms' tumor gene (WT1) expression in B cells and its importance for growth regulation and differentiation. We have investigated WT1 expression in fresh B lymphocytes and in a panel of B-cell lines of normal and malignant origin, including both Epstein-Barr virus (EBV) genome negative and EBV carrying cell lines. WT1 is constitutively activated in all lymphoblastoid cell lines (LCL) derived from EBV immortalization of lymphocytes from normal donors in vitro. These cell lines are distinguished for the presence of activated B-cell markers and an unrestricted expression of viral latent genes. In contrast, WT1 expression is abrogated in normal B lymphocytes and in all Burkitt tumor derived cell lines, irrespective of the EBV genome carrying status and their phenotype pattern. A single step RT-PCR for simultaneous detection of the four spliced transcript isoforms has been applied to confirm their expression. Analysis of variant relative proportions suggested the maintenance of a balanced expression of the isoforms in LCL, as reported in non tumorous tissues. These data, together with the evidence that the replication in vitro of lymphoblastoid cells is not affected by WT1 activation following viral immortalization, support the hypothesis that gene inactivation, in addition to disrupted alternate splicing, may play a role in growth control derangements.
| Original language | English |
|---|---|
| Pages (from-to) | 611-619 |
| Number of pages | 9 |
| Journal | Leukemia and Lymphoma |
| Volume | 38 |
| Issue number | 5-6 |
| Publication status | Published - 2000 |
Fingerprint
Keywords
- B lymphocytes
- BLs
- EBV
- LCL
- WT1
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
Cite this
Wilms' tumor gene expression by normal and malignant human B lymphocytes. / Spinsanti, P.; De Grazia, U.; Faggioni, A.; Frati, L.; Calogero, A.; Ragona, G.
In: Leukemia and Lymphoma, Vol. 38, No. 5-6, 2000, p. 611-619.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Wilms' tumor gene expression by normal and malignant human B lymphocytes
AU - Spinsanti, P.
AU - De Grazia, U.
AU - Faggioni, A.
AU - Frati, L.
AU - Calogero, A.
AU - Ragona, G.
PY - 2000
Y1 - 2000
N2 - Very little is known about Wilms' tumor gene (WT1) expression in B cells and its importance for growth regulation and differentiation. We have investigated WT1 expression in fresh B lymphocytes and in a panel of B-cell lines of normal and malignant origin, including both Epstein-Barr virus (EBV) genome negative and EBV carrying cell lines. WT1 is constitutively activated in all lymphoblastoid cell lines (LCL) derived from EBV immortalization of lymphocytes from normal donors in vitro. These cell lines are distinguished for the presence of activated B-cell markers and an unrestricted expression of viral latent genes. In contrast, WT1 expression is abrogated in normal B lymphocytes and in all Burkitt tumor derived cell lines, irrespective of the EBV genome carrying status and their phenotype pattern. A single step RT-PCR for simultaneous detection of the four spliced transcript isoforms has been applied to confirm their expression. Analysis of variant relative proportions suggested the maintenance of a balanced expression of the isoforms in LCL, as reported in non tumorous tissues. These data, together with the evidence that the replication in vitro of lymphoblastoid cells is not affected by WT1 activation following viral immortalization, support the hypothesis that gene inactivation, in addition to disrupted alternate splicing, may play a role in growth control derangements.
AB - Very little is known about Wilms' tumor gene (WT1) expression in B cells and its importance for growth regulation and differentiation. We have investigated WT1 expression in fresh B lymphocytes and in a panel of B-cell lines of normal and malignant origin, including both Epstein-Barr virus (EBV) genome negative and EBV carrying cell lines. WT1 is constitutively activated in all lymphoblastoid cell lines (LCL) derived from EBV immortalization of lymphocytes from normal donors in vitro. These cell lines are distinguished for the presence of activated B-cell markers and an unrestricted expression of viral latent genes. In contrast, WT1 expression is abrogated in normal B lymphocytes and in all Burkitt tumor derived cell lines, irrespective of the EBV genome carrying status and their phenotype pattern. A single step RT-PCR for simultaneous detection of the four spliced transcript isoforms has been applied to confirm their expression. Analysis of variant relative proportions suggested the maintenance of a balanced expression of the isoforms in LCL, as reported in non tumorous tissues. These data, together with the evidence that the replication in vitro of lymphoblastoid cells is not affected by WT1 activation following viral immortalization, support the hypothesis that gene inactivation, in addition to disrupted alternate splicing, may play a role in growth control derangements.
KW - B lymphocytes
KW - BLs
KW - EBV
KW - LCL
KW - WT1
UR - http://www.scopus.com/inward/record.url?scp=0033867009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033867009&partnerID=8YFLogxK
M3 - Article
C2 - 10953983
AN - SCOPUS:0033867009
VL - 38
SP - 611
EP - 619
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
SN - 1042-8194
IS - 5-6
ER -