Winnie-APCmin/+ mice: A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation

Stefania De Santis; Giulio Verna; Grazia Serino; Raffaele Armentano; Elisabetta Cavalcanti; Marina Liso; Manuela Dicarlo; Sergio Coletta; Mauro Mastronardi; Antonio Lippolis; Angela Tafaro; Angelo Santino; Aldo Pinto; Pietro Campiglia; Alex Y. Huang; Fabio Cominelli; Theresa T. Pizarro; Marcello Chieppa

doi:10.3390/ijms21082972

Winnie-APC^min/+ mice: A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation

Stefania De Santis, Giulio Verna, Grazia Serino, Raffaele Armentano, Elisabetta Cavalcanti, Marina Liso, Manuela Dicarlo, Sergio Coletta, Mauro Mastronardi, Antonio Lippolis, Angela Tafaro, Angelo Santino, Aldo Pinto, Pietro Campiglia, Alex Y. Huang, Fabio Cominelli, Theresa T. Pizarro, Marcello Chieppa

Ente Ospedaliero Specializzato in Gastroenterologia "Saverio de Bellis"

Research output: Contribution to journal › Article › peer-review

Abstract

(1) Background: Colorectal cancer (CRC) is among the best examples of the relationship between inflammation and increased cancer risk. (2) Methods: To examine the effects of spontaneous low-grade chronic inflammation on the pathogenesis of CRC, we developed a new murine model of colitis-associated cancer (CAC) by crossing Mucin 2 mutated mice (Winnie) with Apc^Min/+ mice. (3) Results: The resulting Winnie-Apc^Min/+ model combines an inflammatory background with a genetic predisposition to small intestinal polyposis. Winnie-Apc^Min/+ mice show an early occurrence of inflammatory signs and dysplastic lesions in the distal colon with a specific molecular signature. (4) Conclusion: The Winnie-Apc^Min/+ model is a perfect model to demonstrate that chronic inflammation represents a crucial risk factor for the onset and progression of tumoral lesions in individuals genetically predisposed to CRC.

Original language	English
Article number	2972
Journal	International Journal of Molecular Sciences
Volume	21
Issue number	8
DOIs	https://doi.org/10.3390/ijms21082972
Publication status	Published - Apr 2 2020

Keywords

Aberrant Crypt Foci
Colorectal cancer
Inflammation
Murine model

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

Access to Document

10.3390/ijms21082972

Cite this

De Santis, S., Verna, G., Serino, G., Armentano, R., Cavalcanti, E., Liso, M., Dicarlo, M., Coletta, S., Mastronardi, M., Lippolis, A., Tafaro, A., Santino, A., Pinto, A., Campiglia, P., Huang, A. Y., Cominelli, F., Pizarro, T. T., & Chieppa, M. (2020). Winnie-APC^min/+ mice: A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation. International Journal of Molecular Sciences, 21(8), [2972]. https://doi.org/10.3390/ijms21082972

Winnie-APC^min/+ mice : A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation. / De Santis, Stefania; Verna, Giulio; Serino, Grazia ; Armentano, Raffaele; Cavalcanti, Elisabetta; Liso, Marina; Dicarlo, Manuela; Coletta, Sergio; Mastronardi, Mauro ; Lippolis, Antonio ; Tafaro, Angela; Santino, Angelo; Pinto, Aldo; Campiglia, Pietro; Huang, Alex Y.; Cominelli, Fabio; Pizarro, Theresa T.; Chieppa, Marcello.

In: International Journal of Molecular Sciences, Vol. 21, No. 8, 2972, 02.04.2020.

Research output: Contribution to journal › Article › peer-review

De Santis, S, Verna, G, Serino, G , Armentano, R, Cavalcanti, E, Liso, M, Dicarlo, M, Coletta, S, Mastronardi, M , Lippolis, A , Tafaro, A, Santino, A, Pinto, A, Campiglia, P, Huang, AY, Cominelli, F, Pizarro, TT & Chieppa, M 2020, 'Winnie-APC^min/+ mice: A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation', International Journal of Molecular Sciences, vol. 21, no. 8, 2972. https://doi.org/10.3390/ijms21082972

De Santis, Stefania ; Verna, Giulio ; Serino, Grazia ; Armentano, Raffaele ; Cavalcanti, Elisabetta ; Liso, Marina ; Dicarlo, Manuela ; Coletta, Sergio ; Mastronardi, Mauro ; Lippolis, Antonio ; Tafaro, Angela ; Santino, Angelo ; Pinto, Aldo ; Campiglia, Pietro ; Huang, Alex Y. ; Cominelli, Fabio ; Pizarro, Theresa T. ; Chieppa, Marcello. / Winnie-APC^min/+ mice : A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 8.

@article{9e3cb1c9f02049d78225112ec3522d0f,

title = "Winnie-APCmin/+ mice: A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation",

abstract = "(1) Background: Colorectal cancer (CRC) is among the best examples of the relationship between inflammation and increased cancer risk. (2) Methods: To examine the effects of spontaneous low-grade chronic inflammation on the pathogenesis of CRC, we developed a new murine model of colitis-associated cancer (CAC) by crossing Mucin 2 mutated mice (Winnie) with ApcMin/+ mice. (3) Results: The resulting Winnie-ApcMin/+ model combines an inflammatory background with a genetic predisposition to small intestinal polyposis. Winnie-ApcMin/+ mice show an early occurrence of inflammatory signs and dysplastic lesions in the distal colon with a specific molecular signature. (4) Conclusion: The Winnie-ApcMin/+ model is a perfect model to demonstrate that chronic inflammation represents a crucial risk factor for the onset and progression of tumoral lesions in individuals genetically predisposed to CRC.",

keywords = "Aberrant Crypt Foci, Colorectal cancer, Inflammation, Murine model",

author = "{De Santis}, Stefania and Giulio Verna and Grazia Serino and Raffaele Armentano and Elisabetta Cavalcanti and Marina Liso and Manuela Dicarlo and Sergio Coletta and Mauro Mastronardi and Antonio Lippolis and Angela Tafaro and Angelo Santino and Aldo Pinto and Pietro Campiglia and Huang, {Alex Y.} and Fabio Cominelli and Pizarro, {Theresa T.} and Marcello Chieppa",

note = "Funding Information: Funding: This work was supported by the Italian Ministry of Health GR-2011-02347991, GR-2009-1470633 (G.S.); PO FESR 2014-2020—“Piattaforme tecnologiche di ricerca collaborativa per la lotta alle patologie oncologiche; SiCURA “Soluzioni Innovative per la gestione del paziente e il follow up terapeutico della Colite UlceRosA” (G.S.) and Ricerca Corrente 2019 IRCCS “S. de Bellis” (G.V., E.C., M.L., M.D., S.C., A.T.). S.D.S. is funded by PON -Ricerca e Innovazione 2014-2020-: Progetto AIM1801289 - attivit{\`a} 3 - linea 1. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = apr,

day = "2",

doi = "10.3390/ijms21082972",

language = "English",

volume = "21",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "MDPI AG",

number = "8",

}

TY - JOUR

T1 - Winnie-APCmin/+ mice

T2 - A spontaneous model of colitis-associated colorectal cancer combining genetics and inflammation

AU - De Santis, Stefania

AU - Verna, Giulio

AU - Serino, Grazia

AU - Armentano, Raffaele

AU - Cavalcanti, Elisabetta

AU - Liso, Marina

AU - Dicarlo, Manuela

AU - Coletta, Sergio

AU - Mastronardi, Mauro

AU - Lippolis, Antonio

AU - Tafaro, Angela

AU - Santino, Angelo

AU - Pinto, Aldo

AU - Campiglia, Pietro

AU - Huang, Alex Y.

AU - Cominelli, Fabio

AU - Pizarro, Theresa T.

AU - Chieppa, Marcello

N1 - Funding Information: Funding: This work was supported by the Italian Ministry of Health GR-2011-02347991, GR-2009-1470633 (G.S.); PO FESR 2014-2020—“Piattaforme tecnologiche di ricerca collaborativa per la lotta alle patologie oncologiche; SiCURA “Soluzioni Innovative per la gestione del paziente e il follow up terapeutico della Colite UlceRosA” (G.S.) and Ricerca Corrente 2019 IRCCS “S. de Bellis” (G.V., E.C., M.L., M.D., S.C., A.T.). S.D.S. is funded by PON -Ricerca e Innovazione 2014-2020-: Progetto AIM1801289 - attività 3 - linea 1. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4/2

Y1 - 2020/4/2

N2 - (1) Background: Colorectal cancer (CRC) is among the best examples of the relationship between inflammation and increased cancer risk. (2) Methods: To examine the effects of spontaneous low-grade chronic inflammation on the pathogenesis of CRC, we developed a new murine model of colitis-associated cancer (CAC) by crossing Mucin 2 mutated mice (Winnie) with ApcMin/+ mice. (3) Results: The resulting Winnie-ApcMin/+ model combines an inflammatory background with a genetic predisposition to small intestinal polyposis. Winnie-ApcMin/+ mice show an early occurrence of inflammatory signs and dysplastic lesions in the distal colon with a specific molecular signature. (4) Conclusion: The Winnie-ApcMin/+ model is a perfect model to demonstrate that chronic inflammation represents a crucial risk factor for the onset and progression of tumoral lesions in individuals genetically predisposed to CRC.

AB - (1) Background: Colorectal cancer (CRC) is among the best examples of the relationship between inflammation and increased cancer risk. (2) Methods: To examine the effects of spontaneous low-grade chronic inflammation on the pathogenesis of CRC, we developed a new murine model of colitis-associated cancer (CAC) by crossing Mucin 2 mutated mice (Winnie) with ApcMin/+ mice. (3) Results: The resulting Winnie-ApcMin/+ model combines an inflammatory background with a genetic predisposition to small intestinal polyposis. Winnie-ApcMin/+ mice show an early occurrence of inflammatory signs and dysplastic lesions in the distal colon with a specific molecular signature. (4) Conclusion: The Winnie-ApcMin/+ model is a perfect model to demonstrate that chronic inflammation represents a crucial risk factor for the onset and progression of tumoral lesions in individuals genetically predisposed to CRC.

KW - Aberrant Crypt Foci

KW - Colorectal cancer

KW - Inflammation

KW - Murine model

UR - http://www.scopus.com/inward/record.url?scp=85083823267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85083823267&partnerID=8YFLogxK

U2 - 10.3390/ijms21082972

DO - 10.3390/ijms21082972

M3 - Article

C2 - 32340123

AN - SCOPUS:85083823267

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

M1 - 2972

ER -