Wiskott-Aldrich syndrome protein regulates lipid raft dynamics during immunological synapse formation

Loïc Dupré, Alessandro Aiuti, Sara Trifari, Silvana Martino, Paola Saracco, Claudio Bordignon, Maria Grazia Roncarolo

Research output: Contribution to journalArticle

Abstract

Immunological synapse assembly relies on the clustering of lipid rafts and is required for optimal T cell activation. We demonstrate that the Wiskott-Aldrich syndrome protein (WASP) is recruited to lipid rafts immediately after TCR and CD28 triggering and is required for the movements of lipid rafts. T cells from Wiskott-Aldrich syndrome (WAS) patients, lacking WASP, proliferate poorly after TCR/CD28 activation and have impaired capacities to cluster the lipid raft marker GM1 and to upregulate GM1 cell surface expression. T cell proliferation and lipid raft clustering are restored by retroviral transfer of the WASP gene. These results demonstrate that WASP plays a central role in the movements of lipid rafts and identify a potential mechanism underlying the T cell defect affecting WAS patients.

Original languageEnglish
Pages (from-to)157-166
Number of pages10
JournalImmunity
Volume17
Issue number2
DOIs
Publication statusPublished - Aug 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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    Dupré, L., Aiuti, A., Trifari, S., Martino, S., Saracco, P., Bordignon, C., & Roncarolo, M. G. (2002). Wiskott-Aldrich syndrome protein regulates lipid raft dynamics during immunological synapse formation. Immunity, 17(2), 157-166. https://doi.org/10.1016/S1074-7613(02)00360-6