Abstract
Several studies on disease and treatment effects on neurohormones have been conducted with small numbers of patients, using one blood sample as representative of their states. The aim of this study was to assess the within-patient variability of plasma concentrations of several hormones and cytokines of recent interest, in patients with moderate heart failure and controlled stable background therapy over 3 weeks. Blood for neurohormone and cytokine assays was sampled in duplicate from 18 patients with moderate heart failure. After an initial visit, the patients were kept on stable therapy until the second blood sampling 21 ± 3 days later. The plasma concentrations of several neurohormones (endothelin, renin, angiotensin II, aldosterone, norepinephrine) and cytokines (interleukin-6 (IL-6), interleukin-13 (IL-13), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and soluble receptor type I of tumour necrosis factor-α (sTNF-RI) were measured with immunochemical methods. Some cytokines (IL-13, CNTF and LIF) were not detected. Despite clinically satisfactory ACE inhibition, circulating angiotensin II and aldosterone levels were still elevated in some patients, suggesting aldosterone escape. The between-visit agreement of plasma concentrations measured in duplicate was less than 35% for all circulating factors, except renin which showed a higher variability throughout the 3-week study period.
| Original language | English |
|---|---|
| Pages (from-to) | 213-217 |
| Number of pages | 5 |
| Journal | Pharmacological Research |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1998 |
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Keywords
- Congestive heart failure
- Cytokines
- Hormones
ASJC Scopus subject areas
- Pharmacology
Cite this
Within-patient variability of hormone and cytokine concentrations in heart failure. / Masson, Serge; Latini, Roberto; Bevilacqua, Maurizio; Vago, Tarcisio; Sessa, Fabrizio; Torri, Mauro; Anesini, Adriana; Salio, Monica; Pasotti, Elena; Agnello, Davide; Santoro, Luigi; Catania, Anna; Ghezzi, Pietro; Moccetti, Tiziano; Maggioni, Aldo P.
In: Pharmacological Research, Vol. 37, No. 3, 03.1998, p. 213-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Within-patient variability of hormone and cytokine concentrations in heart failure
AU - Masson, Serge
AU - Latini, Roberto
AU - Bevilacqua, Maurizio
AU - Vago, Tarcisio
AU - Sessa, Fabrizio
AU - Torri, Mauro
AU - Anesini, Adriana
AU - Salio, Monica
AU - Pasotti, Elena
AU - Agnello, Davide
AU - Santoro, Luigi
AU - Catania, Anna
AU - Ghezzi, Pietro
AU - Moccetti, Tiziano
AU - Maggioni, Aldo P.
PY - 1998/3
Y1 - 1998/3
N2 - Several studies on disease and treatment effects on neurohormones have been conducted with small numbers of patients, using one blood sample as representative of their states. The aim of this study was to assess the within-patient variability of plasma concentrations of several hormones and cytokines of recent interest, in patients with moderate heart failure and controlled stable background therapy over 3 weeks. Blood for neurohormone and cytokine assays was sampled in duplicate from 18 patients with moderate heart failure. After an initial visit, the patients were kept on stable therapy until the second blood sampling 21 ± 3 days later. The plasma concentrations of several neurohormones (endothelin, renin, angiotensin II, aldosterone, norepinephrine) and cytokines (interleukin-6 (IL-6), interleukin-13 (IL-13), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and soluble receptor type I of tumour necrosis factor-α (sTNF-RI) were measured with immunochemical methods. Some cytokines (IL-13, CNTF and LIF) were not detected. Despite clinically satisfactory ACE inhibition, circulating angiotensin II and aldosterone levels were still elevated in some patients, suggesting aldosterone escape. The between-visit agreement of plasma concentrations measured in duplicate was less than 35% for all circulating factors, except renin which showed a higher variability throughout the 3-week study period.
AB - Several studies on disease and treatment effects on neurohormones have been conducted with small numbers of patients, using one blood sample as representative of their states. The aim of this study was to assess the within-patient variability of plasma concentrations of several hormones and cytokines of recent interest, in patients with moderate heart failure and controlled stable background therapy over 3 weeks. Blood for neurohormone and cytokine assays was sampled in duplicate from 18 patients with moderate heart failure. After an initial visit, the patients were kept on stable therapy until the second blood sampling 21 ± 3 days later. The plasma concentrations of several neurohormones (endothelin, renin, angiotensin II, aldosterone, norepinephrine) and cytokines (interleukin-6 (IL-6), interleukin-13 (IL-13), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and soluble receptor type I of tumour necrosis factor-α (sTNF-RI) were measured with immunochemical methods. Some cytokines (IL-13, CNTF and LIF) were not detected. Despite clinically satisfactory ACE inhibition, circulating angiotensin II and aldosterone levels were still elevated in some patients, suggesting aldosterone escape. The between-visit agreement of plasma concentrations measured in duplicate was less than 35% for all circulating factors, except renin which showed a higher variability throughout the 3-week study period.
KW - Congestive heart failure
KW - Cytokines
KW - Hormones
UR - http://www.scopus.com/inward/record.url?scp=0032031185&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032031185&partnerID=8YFLogxK
U2 - 10.1006/phrs.1998.0288
DO - 10.1006/phrs.1998.0288
M3 - Article
C2 - 9602470
AN - SCOPUS:0032031185
VL - 37
SP - 213
EP - 217
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 3
ER -