Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing

Giuseppina Sandri, Maria Cristina Bonferoni, Francesca D'Autilia, Silvia Rossi, Franca Ferrari, Pietro Grisoli, Milena Sorrenti, Laura Catenacci, Claudia Del Fante, Cesare Perotti, Carla Caramella

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The management of difficult to heal wounds can considerably reduce the time required for tissue repairing and promote the healing process, minimizing the risk of infection. Silver compounds, especially silver sulfadiazine (AgSD), are often used to prevent or to treat wound colonization, also in presence of antibiotic-resistant bacteria. However, AgSD has been shown to be cytotoxic in vitro toward fibroblasts and keratinocytes and consequently to retard wound healing in vivo. Recently, platelet lysate (PL) has been proposed in clinical practice for the healing of persistent lesions. The aim of the present work was the development of wound dressings based on AgSD loaded in solid lipid nanoparticles (SLNs), to be used in association with PL for the treatment for skin lesions. SLN were based on chondroitin sulfate and sodium hyaluronate, bioactive polymers characterized by well-known tissue repairing properties. The encapsulation of AgSD in SLN aimed at preventing the cytotoxic effect of the drug on normal human dermal fibroblasts (NHDFs) and at enabling the association of the drug with PL. SLN were loaded in wound dressings based on hydroxypropylmethyl cellulose (HPMC) or chitosan glutamate (CS glu). These polymers were chosen to obtain a sponge matrix with suitable elasticity and softness and, moreover, with good bioadhesive behavior on skin lesions. Dressings based on chitosan glutamate showed antimicrobial activity with and without PL. Even though further in vivo evaluation could be envisaged, chitosan based dressings demonstrated to be a suitable prototype for the treatment for skin lesions.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume84
Issue number1
DOIs
Publication statusPublished - May 2013

Fingerprint

Silver Sulfadiazine
Bandages
Nanoparticles
Chitosan
Blood Platelets
Lipids
Skin
Wounds and Injuries
Glutamic Acid
Silver Compounds
Polymers
Fibroblasts
Elasticity
Porifera
Keratinocytes
Cellulose
Pharmaceutical Preparations
Wound Healing
Anti-Bacterial Agents
Bacteria

Keywords

  • Platelet lysate
  • Silver sulfadiazine
  • Skin lesions
  • SLN
  • Wound healing

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

Cite this

Sandri, G., Bonferoni, M. C., D'Autilia, F., Rossi, S., Ferrari, F., Grisoli, P., ... Caramella, C. (2013). Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing. European Journal of Pharmaceutics and Biopharmaceutics, 84(1), 84-90. https://doi.org/10.1016/j.ejpb.2012.11.022

Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing. / Sandri, Giuseppina; Bonferoni, Maria Cristina; D'Autilia, Francesca; Rossi, Silvia; Ferrari, Franca; Grisoli, Pietro; Sorrenti, Milena; Catenacci, Laura; Del Fante, Claudia; Perotti, Cesare; Caramella, Carla.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 84, No. 1, 05.2013, p. 84-90.

Research output: Contribution to journalArticle

Sandri, Giuseppina ; Bonferoni, Maria Cristina ; D'Autilia, Francesca ; Rossi, Silvia ; Ferrari, Franca ; Grisoli, Pietro ; Sorrenti, Milena ; Catenacci, Laura ; Del Fante, Claudia ; Perotti, Cesare ; Caramella, Carla. / Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing. In: European Journal of Pharmaceutics and Biopharmaceutics. 2013 ; Vol. 84, No. 1. pp. 84-90.
@article{9c6a983ba6de4d508234fff27464e200,
title = "Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing",
abstract = "The management of difficult to heal wounds can considerably reduce the time required for tissue repairing and promote the healing process, minimizing the risk of infection. Silver compounds, especially silver sulfadiazine (AgSD), are often used to prevent or to treat wound colonization, also in presence of antibiotic-resistant bacteria. However, AgSD has been shown to be cytotoxic in vitro toward fibroblasts and keratinocytes and consequently to retard wound healing in vivo. Recently, platelet lysate (PL) has been proposed in clinical practice for the healing of persistent lesions. The aim of the present work was the development of wound dressings based on AgSD loaded in solid lipid nanoparticles (SLNs), to be used in association with PL for the treatment for skin lesions. SLN were based on chondroitin sulfate and sodium hyaluronate, bioactive polymers characterized by well-known tissue repairing properties. The encapsulation of AgSD in SLN aimed at preventing the cytotoxic effect of the drug on normal human dermal fibroblasts (NHDFs) and at enabling the association of the drug with PL. SLN were loaded in wound dressings based on hydroxypropylmethyl cellulose (HPMC) or chitosan glutamate (CS glu). These polymers were chosen to obtain a sponge matrix with suitable elasticity and softness and, moreover, with good bioadhesive behavior on skin lesions. Dressings based on chitosan glutamate showed antimicrobial activity with and without PL. Even though further in vivo evaluation could be envisaged, chitosan based dressings demonstrated to be a suitable prototype for the treatment for skin lesions.",
keywords = "Platelet lysate, Silver sulfadiazine, Skin lesions, SLN, Wound healing",
author = "Giuseppina Sandri and Bonferoni, {Maria Cristina} and Francesca D'Autilia and Silvia Rossi and Franca Ferrari and Pietro Grisoli and Milena Sorrenti and Laura Catenacci and {Del Fante}, Claudia and Cesare Perotti and Carla Caramella",
year = "2013",
month = "5",
doi = "10.1016/j.ejpb.2012.11.022",
language = "English",
volume = "84",
pages = "84--90",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing

AU - Sandri, Giuseppina

AU - Bonferoni, Maria Cristina

AU - D'Autilia, Francesca

AU - Rossi, Silvia

AU - Ferrari, Franca

AU - Grisoli, Pietro

AU - Sorrenti, Milena

AU - Catenacci, Laura

AU - Del Fante, Claudia

AU - Perotti, Cesare

AU - Caramella, Carla

PY - 2013/5

Y1 - 2013/5

N2 - The management of difficult to heal wounds can considerably reduce the time required for tissue repairing and promote the healing process, minimizing the risk of infection. Silver compounds, especially silver sulfadiazine (AgSD), are often used to prevent or to treat wound colonization, also in presence of antibiotic-resistant bacteria. However, AgSD has been shown to be cytotoxic in vitro toward fibroblasts and keratinocytes and consequently to retard wound healing in vivo. Recently, platelet lysate (PL) has been proposed in clinical practice for the healing of persistent lesions. The aim of the present work was the development of wound dressings based on AgSD loaded in solid lipid nanoparticles (SLNs), to be used in association with PL for the treatment for skin lesions. SLN were based on chondroitin sulfate and sodium hyaluronate, bioactive polymers characterized by well-known tissue repairing properties. The encapsulation of AgSD in SLN aimed at preventing the cytotoxic effect of the drug on normal human dermal fibroblasts (NHDFs) and at enabling the association of the drug with PL. SLN were loaded in wound dressings based on hydroxypropylmethyl cellulose (HPMC) or chitosan glutamate (CS glu). These polymers were chosen to obtain a sponge matrix with suitable elasticity and softness and, moreover, with good bioadhesive behavior on skin lesions. Dressings based on chitosan glutamate showed antimicrobial activity with and without PL. Even though further in vivo evaluation could be envisaged, chitosan based dressings demonstrated to be a suitable prototype for the treatment for skin lesions.

AB - The management of difficult to heal wounds can considerably reduce the time required for tissue repairing and promote the healing process, minimizing the risk of infection. Silver compounds, especially silver sulfadiazine (AgSD), are often used to prevent or to treat wound colonization, also in presence of antibiotic-resistant bacteria. However, AgSD has been shown to be cytotoxic in vitro toward fibroblasts and keratinocytes and consequently to retard wound healing in vivo. Recently, platelet lysate (PL) has been proposed in clinical practice for the healing of persistent lesions. The aim of the present work was the development of wound dressings based on AgSD loaded in solid lipid nanoparticles (SLNs), to be used in association with PL for the treatment for skin lesions. SLN were based on chondroitin sulfate and sodium hyaluronate, bioactive polymers characterized by well-known tissue repairing properties. The encapsulation of AgSD in SLN aimed at preventing the cytotoxic effect of the drug on normal human dermal fibroblasts (NHDFs) and at enabling the association of the drug with PL. SLN were loaded in wound dressings based on hydroxypropylmethyl cellulose (HPMC) or chitosan glutamate (CS glu). These polymers were chosen to obtain a sponge matrix with suitable elasticity and softness and, moreover, with good bioadhesive behavior on skin lesions. Dressings based on chitosan glutamate showed antimicrobial activity with and without PL. Even though further in vivo evaluation could be envisaged, chitosan based dressings demonstrated to be a suitable prototype for the treatment for skin lesions.

KW - Platelet lysate

KW - Silver sulfadiazine

KW - Skin lesions

KW - SLN

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84876942241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876942241&partnerID=8YFLogxK

U2 - 10.1016/j.ejpb.2012.11.022

DO - 10.1016/j.ejpb.2012.11.022

M3 - Article

C2 - 23207329

AN - SCOPUS:84876942241

VL - 84

SP - 84

EP - 90

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

IS - 1

ER -