TY - JOUR
T1 - Wound Healing Fluid Reflects the Inflammatory Nature and Aggressiveness of Breast Tumors
AU - Agresti, Roberto
AU - Triulzi, Tiziana
AU - Sasso, Marianna
AU - Ghirelli, Cristina
AU - Aiello, Piera
AU - Rybinska, Ilona
AU - Campiglio, Manuela
AU - Sfondrini, Lucia
AU - Tagliabue, Elda
AU - Bianchi, Francesca
PY - 2019/2/19
Y1 - 2019/2/19
N2 - Wound healing fluid that originates from breast surgery increases the aggressiveness of cancer cells that remain after the surgery. We determined the effects of the extent of surgery and tumor-driven remodeling of the surrounding microenvironment on the ability of wound-healing to promote breast cancer progression. In our analysis of a panel of 34 cytokines, chemokines, and growth factors in wound healing fluid, obtained from 27 breast carcinoma patients after surgery, the levels of several small molecules were associated with the extent of cellular damage that was induced by surgery. In addition, the composition of the resulting wound healing fluid was associated with molecular features of the removed tumor. Specifically, IP-10, IL-6, G-CSF, osteopontin, MIP-1a, MIP-1b, and MCP1-MCAF were higher in more aggressive tumors. Altogether, our findings indicate that the release of factors that are induced by removal of the primary tumor and subsequent wound healing is influenced by the extent of damage due to surgery and the reactive stroma that is derived from the continuously evolving network of interactions between neoplastic cells and the microenvironment, based on the molecular characteristics of breast carcinoma cells.
AB - Wound healing fluid that originates from breast surgery increases the aggressiveness of cancer cells that remain after the surgery. We determined the effects of the extent of surgery and tumor-driven remodeling of the surrounding microenvironment on the ability of wound-healing to promote breast cancer progression. In our analysis of a panel of 34 cytokines, chemokines, and growth factors in wound healing fluid, obtained from 27 breast carcinoma patients after surgery, the levels of several small molecules were associated with the extent of cellular damage that was induced by surgery. In addition, the composition of the resulting wound healing fluid was associated with molecular features of the removed tumor. Specifically, IP-10, IL-6, G-CSF, osteopontin, MIP-1a, MIP-1b, and MCP1-MCAF were higher in more aggressive tumors. Altogether, our findings indicate that the release of factors that are induced by removal of the primary tumor and subsequent wound healing is influenced by the extent of damage due to surgery and the reactive stroma that is derived from the continuously evolving network of interactions between neoplastic cells and the microenvironment, based on the molecular characteristics of breast carcinoma cells.
KW - Body Fluids/metabolism
KW - Breast Neoplasms/pathology
KW - Cell Line, Tumor
KW - Cell Movement
KW - Cell Proliferation
KW - Female
KW - Humans
KW - Inflammation/pathology
KW - Neoplasm Invasiveness
KW - Triple Negative Breast Neoplasms/pathology
KW - Wound Healing
U2 - 10.3390/cells8020181
DO - 10.3390/cells8020181
M3 - Article
C2 - 30791501
VL - 8
JO - Cells
JF - Cells
SN - 2073-4409
IS - 2
ER -