WT1 CpG islands methylation in human lung cancer: A pilot study

Pierdonato Bruno, Giovanna Gentile, Rita Mancini, Claudia de Vitis, Maria Cristina Esposito, Davide Scozzi, Mario Mastrangelo, Alberto Ricci, Ibrahim Mohsen, Gennaro Ciliberto, Maurizio Simmaco, Salvatore Mariotta

Research output: Contribution to journalArticlepeer-review

Abstract

Background: CpG island hypermethylation of gene promoters and regulatory regions is a well-known mechanism of epigenetic silencing of tumor suppressors and is directly linked to carcinogenesis. Wilm's tumor gene (WT1) is a tumor suppressor protein involved in the regulation of human cell growth and differentiation and a modulator of oncogenic K Ras signaling in lung cancer. Changes in the pattern of methylation of the WT1 gene have not yet been studied in detail in human lung cancer. In this study we compared the methylation profile of WT1 gene in samples of neoplastic and non-neoplastic lung tissue taken from the same patients. Methods: DNA was extracted from neoplastic and normal lung tissue obtained from 16 patients with non small cell lung cancer (NSCLC). The methylation status of 29 CpG islands in the 5' region of WT1 was determined by pyrosequencing. Statistical analysis was carried out by T test and Mann Whitney test. Results: The mean percentage of methylation, considering all CpG islands of WT1 in the neoplastic tissues of the 16 NSCLC patients, was 16.2 ± 3.4, whereas in the normal lung tissue from the same patients it was 5.6 ± 1.7 (p

Original languageEnglish
Pages (from-to)306-309
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume426
Issue number3
DOIs
Publication statusPublished - Sep 28 2012

Keywords

  • Gene
  • Human
  • Lung cancer
  • Methylation
  • NSCLC
  • WT1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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