X chromosome monosomy: A common mechanism for autoimmune diseases

Pietro Invernizzi, Monica Miozzo, Carlo Selmi, Luca Persani, Pier Maria Battezzati, Massimo Zuin, Simona Lucchi, Pier Luigi Meroni, Bianca Marasini, Silvana Zeni, Mitchell Watnik, Francesca R. Grati, Giuseppe Simoni, M. Eric Gershwin, Mauro Podda

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The majority of human autoimmune diseases are characterized by female predominance. Although sex hormone influences have been suggested to explain this phenomenon, the mechanism remains unclear. In contrast to the role of hormones, it has been suggested, based on pilot data in primary biliary cirrhosis, that there is an elevation of monosomy X in autoimmune disease. Using peripheral white blood cells from women with systemic sclerosis (SSc), autoimmune thyroid disease (AITD), or healthy age-matched control women, we studied the presence of monosomy X rates using fluorescence in situ hybridization. We also performed dual-color fluorescence in situ hybridization analysis with a chromosome Y α-satellite probe to determine the presence of the Y chromosome in the monosomic cells. In subsets of patients and controls, we determined X monosomy rates in white blood cell subpopulations. The rates of monosomy X increased with age in all three populations. However, the rate of monosomy X was significantly higher in patients with SSc and AITD when compared with healthy women (6.2 ± 0.3% and 4.3 ± 0.3%, respectively, vs 2.9 ± 0.2% in healthy women, p <0.0001 in both comparisons). Importantly, X monosomy rate was more frequent in peripheral T and B lymphocytes than in the other blood cell populations, and there was no evidence for the presence of male fetal microchimerism. These data highlight the thesis that chromosome instability is common to women with SSc and AITD and that haploinsufficiency for X-linked genes may be a critical factor for the female predominance of autoimmune diseases.

Original languageEnglish
Pages (from-to)575-578
Number of pages4
JournalJournal of Immunology
Volume175
Issue number1
Publication statusPublished - Jul 1 2005

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Monosomy
Turner Syndrome
X Chromosome
Autoimmune Diseases
Systemic Scleroderma
Thyroid Diseases
Y Chromosome
Fluorescence In Situ Hybridization
Leukocytes
Haploinsufficiency
X-Linked Genes
Chromosomal Instability
Chimerism
Biliary Liver Cirrhosis
Gonadal Steroid Hormones
Population
Blood Cells
B-Lymphocytes
Color
Hormones

ASJC Scopus subject areas

  • Immunology

Cite this

X chromosome monosomy : A common mechanism for autoimmune diseases. / Invernizzi, Pietro; Miozzo, Monica; Selmi, Carlo; Persani, Luca; Battezzati, Pier Maria; Zuin, Massimo; Lucchi, Simona; Meroni, Pier Luigi; Marasini, Bianca; Zeni, Silvana; Watnik, Mitchell; Grati, Francesca R.; Simoni, Giuseppe; Gershwin, M. Eric; Podda, Mauro.

In: Journal of Immunology, Vol. 175, No. 1, 01.07.2005, p. 575-578.

Research output: Contribution to journalArticle

Invernizzi, P, Miozzo, M, Selmi, C, Persani, L, Battezzati, PM, Zuin, M, Lucchi, S, Meroni, PL, Marasini, B, Zeni, S, Watnik, M, Grati, FR, Simoni, G, Gershwin, ME & Podda, M 2005, 'X chromosome monosomy: A common mechanism for autoimmune diseases', Journal of Immunology, vol. 175, no. 1, pp. 575-578.
Invernizzi, Pietro ; Miozzo, Monica ; Selmi, Carlo ; Persani, Luca ; Battezzati, Pier Maria ; Zuin, Massimo ; Lucchi, Simona ; Meroni, Pier Luigi ; Marasini, Bianca ; Zeni, Silvana ; Watnik, Mitchell ; Grati, Francesca R. ; Simoni, Giuseppe ; Gershwin, M. Eric ; Podda, Mauro. / X chromosome monosomy : A common mechanism for autoimmune diseases. In: Journal of Immunology. 2005 ; Vol. 175, No. 1. pp. 575-578.
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