X-ray crystal structure of the ferric sperm whale myoglobin: Imidazole complex at 2.0 Å resolution

Claudia Lionetti, Maria Grazia Guanziroli, Francesco Frigerio, Paolo Ascenzi, Martino Bolognesi

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Abstract

The X-ray crystal structure of the ferric sperm whale (Physeter catodon) myoglobin: imidazole complex has been refined at 2.0 Å resolution, to a final R-factor of 14.8%. The overall conformation of the protein is little affected by binding of the ligand. Imidazole is co-ordinated to the heme iron at the distal site, and forces distinguishable local changes in the surrounding protein residues. His64(E7) swings out of the distal pocket and becomes substantially exposed to the solvent; nevertheless, it stabilizes the exogenous ligand by hydrogen bonding. The side-chains of residues Arg45(CD3) and Asp60(E3) are also affected by imidazole association.

Original languageEnglish
Pages (from-to)409-412
Number of pages4
JournalJournal of Molecular Biology
Volume217
Issue number3
DOIs
Publication statusPublished - Feb 5 1991

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Sperm Whale
Myoglobin
X-Rays
R388
Ligands
Protein Conformation
Hydrogen Bonding
Heme
Iron
imidazole
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

X-ray crystal structure of the ferric sperm whale myoglobin : Imidazole complex at 2.0 Å resolution. / Lionetti, Claudia; Guanziroli, Maria Grazia; Frigerio, Francesco; Ascenzi, Paolo; Bolognesi, Martino.

In: Journal of Molecular Biology, Vol. 217, No. 3, 05.02.1991, p. 409-412.

Research output: Contribution to journalArticle

Lionetti, Claudia ; Guanziroli, Maria Grazia ; Frigerio, Francesco ; Ascenzi, Paolo ; Bolognesi, Martino. / X-ray crystal structure of the ferric sperm whale myoglobin : Imidazole complex at 2.0 Å resolution. In: Journal of Molecular Biology. 1991 ; Vol. 217, No. 3. pp. 409-412.
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