X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis

Ginevra Begani Provinciali, Nicola Pieroni, Inna Bukreeva, Michela Fratini, Lorenzo Massimi, Laura Maugeri, Francesca Palermo, Fabrizio Bardelli, Alberto Mittone, Alberto Bravin, Giuseppe Gigli, Francesco Gentile, Andrea Fossaghi, Nilo Riva, Angelo Quattrini, Alessia Cedola

Research output: Contribution to journalArticlepeer-review

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting motor neurons. Pre-clinical studies drive the development of animal models that well mimic ALS disorder and enable both the dissection of disease processes and an early assessment of therapy efficacy. A comprehensive knowledge of neuronal and vascular lesions in the brain and spinal cord is an essential factor to understand the development of the disease. Spatial resolution and bidimensional imaging are important drawbacks limiting current neuroimaging tools, while neuropathology relies on protocols that may alter tissue chemistry and structure. In contrast, recent ex vivo studies in mice demonstrated that X-ray phase-contrast tomography enables study of the 3D distribution of both vasculature and neuronal networks, without sample sectioning or use of staining. Here we present our findings on ex vivo SOD1G93A ALS mice spinal cord at a micrometric scale. An unprecedented direct quantification of neuro-vascular alterations at different stages of the disease is shown.

Original languageEnglish
Pages (from-to)1042-1048
Number of pages7
JournalJournal of Synchrotron Radiation
Volume27
DOIs
Publication statusPublished - Jul 1 2020

Keywords

  • ALS
  • spinal cord
  • X-ray phase contrast tomography

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

Fingerprint Dive into the research topics of 'X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis'. Together they form a unique fingerprint.

Cite this