XRF analyses reveal that capacitation procedures produce changes in magnesium and copper levels in human sperm

Lorella Pascolo, Luisa Zupin, Alessandra Gianoncelli, Elena Giolo, Stefania Luppi, Monica Martinelli, Daniela De Rocco, Simone Sala, Sergio Crovella, Giuseppe Ricci

Research output: Contribution to journalArticlepeer-review

Abstract

Capacitation makes spermatozoa competent to fertilize oocytes and can be induced in vitro before Artificial Reproductive Technology (ART) procedures to select the best quality and motile spermatozoa. The sperm quality is routinely measured under light microscopy. In the present study we tested the possibility of adding quality information by evaluating the content of some light elements and metals by XRF imaging, similarly to what we previously performed for female primordial gametes. With this approach we identified some major differences in elemental concentration between capacitated and not capacitated semen, indicating that Mg and Cu sperm contents are greatly reduced by capacitation, while there is an apparent Na increase.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume459
DOIs
Publication statusPublished - Nov 15 2019

Keywords

  • Capacitation
  • Sperm
  • STXM
  • XRF

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

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