YAP1 acts as oncogenic target of 11q22 amplification in multiple cancer subtypes

Erica Lorenzetto, Monica Brenca, Mattia Boeri, Carla Verri, Elena Piccinin, Patrizia Gasparini, Federica Facchinetti, Sabrina Rossi, Giuliana Salvatore, Maura Massimino, Gabriella Sozzi, Roberta Maestro, Piergiorgio Modena

Research output: Contribution to journalArticle

Abstract

The transcriptional coactivator YAP1 is a critical effector of the human Salvador-Warts-Hippo pathway. Literature data report apparently discrepant results on the carcinogenic role of YAP1, which acts either as oncogene or as tumor suppressor in different in vitro and in vivo models. Furthermore, genomic amplification events of 11q22 locus encompassing YAP1 gene have been detected in multiple tumor types but there is limited direct evidence about the oncogenic role of endogenous YAP1 within in the amplicon. We screened a panel of human tumor samples and cancer cell lines and identified that the YAP1 amplification event is actually present in up to 23% of the cases. We exploited EKVX (lung cancer), CaSki (cervical cancer) and RO82 (thyroid cancer) cell lines harboring both genomic YAP1 amplification and YAP1 protein overexpression, in order to study the effects of downregulation of endogenous YAP1 by RNA-interference strategies. Class comparison analysis of gene expression profiling data identified 707 statistically significantly modulated genes (multivariable global test p-value = 0.002) that were functionally annotated for cell proliferation and cellular movement ontologies. Mechanistic studies of the identified perturbed pathways revealed that YAP1 silencing significantly decreased cell proliferation and cell cycle perturbation associated with upregulation of p21 and p27 cell-cycle inhibitors, reduced cell migration (p

Original languageEnglish
Pages (from-to)2608-2621
Number of pages14
JournalOncotarget
Volume5
Issue number9
Publication statusPublished - 2014

Keywords

  • Cervical cancer
  • Gene amplification
  • Lung cancer
  • Oncogene
  • Salvador-Warts-Hippo pathway
  • YAP1

ASJC Scopus subject areas

  • Oncology
  • Medicine(all)

Fingerprint Dive into the research topics of 'YAP1 acts as oncogenic target of 11q22 amplification in multiple cancer subtypes'. Together they form a unique fingerprint.

  • Cite this

    Lorenzetto, E., Brenca, M., Boeri, M., Verri, C., Piccinin, E., Gasparini, P., Facchinetti, F., Rossi, S., Salvatore, G., Massimino, M., Sozzi, G., Maestro, R., & Modena, P. (2014). YAP1 acts as oncogenic target of 11q22 amplification in multiple cancer subtypes. Oncotarget, 5(9), 2608-2621.