YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression

Karsten Jürchott, Stephan Bergmann, Ulrike Stein, Wolfgang Walther, Martin Janz, Isabella Manni, Giulia Piaggio, Ellen Fietze, Manfred Dietel, Hans Dieter Royer

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of the Y-box protein YB-1 is increased in proliferating normal and cancer cells, but its role in cell proliferation and cell cycle progression is unclear. We have identified a cell cycle-dependent relocalization of YB-1 from the cytoplasm to the nucleus at the G1/S phase transition and demonstrate that both the charged zipper and the cold shock domain are involved in regulating this process. Using cell lines that constitutively overexpress YB-1, we show that nuclear accumulation of YB-1 is associated with increased cyclin A and cyclin B1 mRNA and protein expression. We provide evidence that deregulated YB-1 expression is linked to adhesion-independent cell proliferation through the induction of cyclin A. Thus, we have identified YB-1 as a cell cycle stage-specific transcription factor important for cell proliferation.

Original languageEnglish
Pages (from-to)27988-27996
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number30
DOIs
Publication statusPublished - Jul 25 2003

ASJC Scopus subject areas

  • Biochemistry

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