Yeast and mammalian metabolism continuous monitoring by pressure recording as an assessment technique for xenobiotic agent effects

M. Milani, M. Ballerini, L. Ferraro, E. Marelli, F. Mazza, M. Zabeo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our work is devoted to the Study of Saccharomyces cerevisiae and human lymphocytes cellular metabolism in order to develop a reference model to assess biological systems responses to chemical or physical agents exposure. CO 2 variations inside test-tubes are measured by differential pressure sensors; pressure values are subsequently convened in voltage. The system allows to test up to 16 samples at the same time. Sampling manages up to 100 acquisitions per second. Values are recorded by a data acquisition card connected to a computer. This procedure leads to a standard curve (pressure variation versus time), typical of the cellular line, that describe cellular metabolism. The longest time lapse used is of 170 h. Different phases appear in this curve: an initial growth up to a maximum, followed by a decrement that leads to a typical "depression" (pressure value inside the test-tubes is lower than the initial one) after about 35 h from the beginning in the case of yeast cells. The curve is reproducible within an experimental error of 4%. The analysis of many samples and the low cost of the devices allow a good statistical significance of the data. In particular as a test we will compare two sterilising agents effects: UV radiation and amuchina®.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsG.E. Cohn
Pages44-55
Number of pages12
Volume4625
DOIs
Publication statusPublished - 2002
EventClinical Diagnostic Systems: Technologies and Instrumentation - San Jose, CA, United States
Duration: Jan 22 2002Jan 24 2002

Other

OtherClinical Diagnostic Systems: Technologies and Instrumentation
CountryUnited States
CitySan Jose, CA
Period1/22/021/24/02

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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  • Cite this

    Milani, M., Ballerini, M., Ferraro, L., Marelli, E., Mazza, F., & Zabeo, M. (2002). Yeast and mammalian metabolism continuous monitoring by pressure recording as an assessment technique for xenobiotic agent effects. In G. E. Cohn (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4625, pp. 44-55) https://doi.org/10.1117/12.469775