Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells

Pietro Vella, Iros Barozzi, Alessandro Cuomo, Tiziana Bonaldi, Diego Pasini

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and mediates Polycomb group proteins (PcG) recruitment to DNA. Despite several publications pointing at YY1 having a similar role in mammalians, others showed features of YY1 that are not compatible with PcG functions. Here, we show that, in mouse Embryonic Stem (ES) cells, YY1 has genome-wide PcG-independent activities while it is still stably associated with the INO80 chromatin-remodeling complex, as well as with novel RNA helicase activities. YY1 binds chromatin in close proximity of the transcription start site of highly expressed genes. Loss of YY1 functions preferentially led to a down-regulation of target genes expression, as well as to an up-regulation of several small non-coding RNAs, suggesting a role for YY1 in regulating small RNA biogenesis. Finally, we found that YY1 is a novel player of Myc-related transcription factors and that its coordinated binding at promoters potentiates gene expression, proposing YY1 as an active component of the Myc transcription network that links ES to cancer cells.

Original languageEnglish
Pages (from-to)3403-3418
Number of pages16
JournalNucleic Acids Research
Volume40
Issue number8
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Yin-Yang
Embryonic Stem Cells
Transcription Factors
Polycomb-Group Proteins
RNA Helicases
Small Untranslated RNA
Chromatin Assembly and Disassembly
Neoplastic Stem Cells
Transcription Initiation Site
DNA
Gene Expression Regulation
Response Elements
Drosophila melanogaster
Chromatin
Embryonic Development
Publications
Up-Regulation
Down-Regulation

ASJC Scopus subject areas

  • Genetics

Cite this

Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells. / Vella, Pietro; Barozzi, Iros; Cuomo, Alessandro; Bonaldi, Tiziana; Pasini, Diego.

In: Nucleic Acids Research, Vol. 40, No. 8, 04.2012, p. 3403-3418.

Research output: Contribution to journalArticle

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