Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello, Luca Cardone, Corrado Garbi, Michael D. Ginsberg, Stelio Varrone, Charles S. Rubin, Enrico V. Avvedimento, Max E. Gottesman

Research output: Contribution to journalArticle

Abstract

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalFEBS Letters
Volume464
Issue number3
DOIs
Publication statusPublished - Dec 31 1999

Keywords

  • NMDA receptor
  • Protein kinase A
  • Yotiao protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Feliciello, A., Cardone, L., Garbi, C., Ginsberg, M. D., Varrone, S., Rubin, C. S., Avvedimento, E. V., & Gottesman, M. E. (1999). Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II. FEBS Letters, 464(3), 174-178. https://doi.org/10.1016/S0014-5793(99)01585-9