ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor

R. Zanotti, A. Ambrosetti, M. Lestani, P. Ghia, C. Pattaro, A. Remo, F. Zanetti, S. Stella, O. Perbellini, G. Prato, G. Guida, F. Caligaris-Cappio, F. Menestrina, G. Pizzolo, M. Chilosi

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Abstract

Zeta-associated protein-70 (ZAP-70), mostly assessed by flow-cytometry (FC), recently emerged as reliable prognostic factor in chronic lymphocytic leukaemia (CLL) at presentation. We evaluated ZAP-70 expression in 156 CLL patients by immunohistochemistry (IHC) on formalin-fixed bone marrow (BM) biopsies at diagnosis. At presentation, 117 patients (75%) were with Binet stage A, 27 (17%) stage B and 12 (8%) stage C. Median follow-up was 61 months (range 6-242). ZAP-70 was expressed in neoplastic lymphocytes of 69 patients (44%). Concordance between ZAP-70 by IHC and ZAP-70 by FC, immunoglobulin heavy chain variable genes (IGHV) mutational status and CD38 expression was found in 41/46 (89%), 41/49 (80%) and in 60/88 (68%) tested cases, respectively. ZAP-70 expression significantly correlated with advanced Binet stage (B-C), diffuse BM infiltration, increased lactate dehydrogenase (LDH) and β2-microglobulin serum levels and lymphocyte doubling time

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalLeukemia
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2007

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ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Zanotti, R., Ambrosetti, A., Lestani, M., Ghia, P., Pattaro, C., Remo, A., Zanetti, F., Stella, S., Perbellini, O., Prato, G., Guida, G., Caligaris-Cappio, F., Menestrina, F., Pizzolo, G., & Chilosi, M. (2007). ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor. Leukemia, 21(1), 102-109. https://doi.org/10.1038/sj.leu.2404458