ZFP423 coordinates Notch and bone morphogenetic protein signaling, selectively up-regulating Hes5 gene expression

Giacomo Masserdotti, Aurora Badaloni, Yangsook Song Green, Laura Croci, Valeria Barili, Giorgio Bergamini, Monica L. Vetter, G. Giacomo Consalez

Research output: Contribution to journalArticlepeer-review

Abstract

Zinc finger protein 423 encodes a 30 Zn-finger transcription factor involved in cerebellar and olfactory development. ZFP423 is a known interactor of SMAD1-SMAD4 and of Collier/Olf-1/EBF proteins, and acts as a modifier of retinoic acid-induced differentiation. In the present article, we show that ZFP423 interacts with the Notch1 intracellular domain in mammalian cell lines and in Xenopus neurula embryos, to activate the expression of the Notch1 target Hes5/ESR1. This effect is antagonized by EBF transcription factors, both in cultured cells and in Xenopus embryos, and amplified in vitro by BMP4, suggesting that ZFP423 acts to integrate BMP and Notch signaling, selectively promoting their convergence onto the Hes5 gene promoter.

Original languageEnglish
Pages (from-to)30814-30824
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number40
DOIs
Publication statusPublished - Oct 1 2010

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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