Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

Marie Perez, Giovanni Rompato, Nicoletta Corbi, Laura De Gregorio, Tommaso A. Dragani, Claudio Passananti

Research output: Contribution to journalArticle

Abstract

The complete cDNA coding sequence of the zinc finger gene Zfp60 is reported. The predicted amino acid sequence of the Zfp60 protein has been found to contain 19 zinc finger motives clustered at the C-terminus. At its N-terminus, Zfp60 shares with other members of the zinc finger gene family two additional conserved amino acid modules named Kruppel Associated Boxes (KRAB). The expression patterns of Zfp60, MyoD and MHC mRNAs have been followed during in vitro myogenic differentiation of C2 cells. We show that the bacterial produced Zfp60 protein binds DNA only in presence of zinc ions, Zfp60 locus has been mapped in chromosome 7, where other Zfp loci are localised.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalFEBS Letters
Volume387
Issue number2-3
DOIs
Publication statusPublished - Jun 3 1996

Keywords

  • cDNA cloning
  • Chromosome mapping
  • DNA binding
  • Myogenic differentiation
  • Temporal expression
  • Zinc finger protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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