Zinc induces apoptosis of human melanoma cells, increasing reactive oxygen species, p53 and FAS ligand

Mauro Provinciali, Elisa Pierpaoli, Beatrice Bartozzi, Giovanni Bernardini

Research output: Contribution to journalArticle

Abstract

The aim of this study was to examine the in vitro effect of zinc on the apoptosis of human melanoma cells, by studying the zinc-dependent modulation of intracellular levels of reactive oxygen species (ROS) and of p53 and FAS ligand proteins. We showed that zinc concentrations ranging from 33.7 μM to 75 μM Zn2+ induced apoptosis in the human melanoma cell line WM 266-4. This apoptosis was associated with an increased production of intracellular ROS, and of p53 and FAS ligand protein. Treatment of tumor cells with the antioxidant N-acetylcysteine was able to prevent Zn2+-induced apoptosis, as well as the increase of p53 and FAS ligand protein induced by zinc. Zinc induces apoptosis in melanoma cells by increasing ROS and this effect may be mediated by the ROS-dependent induction of p53 and FAS/FAS ligand.

Original languageEnglish
Pages (from-to)5309-5316
Number of pages8
JournalAnticancer Research
Volume35
Issue number10
Publication statusPublished - Oct 1 2015

Keywords

  • Apoptosis
  • FAS ligand
  • Melanoma
  • P53
  • Reactive oxygen species
  • Zinc

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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