ZNF75: Isolation of a cDNA clone of the KRAB zinc finger gene subfamily mapped in YACs 1 Mb telomeric of HPRT

A. Villa, I. Zucchi, G. Pilia, D. Strina, L. Susani, F. Morali, C. Patrosso, A. Frattini, F. Lucchini, M. Repetto, M. G. Sacco, M. Zoppe, P. Vezzoni

Research output: Contribution to journalArticle

Abstract

We have previously mapped a zinc finger genomic motif (ZNF75) to the Xq26 cytogenetic band by using a hybrid panel. Here, we report the isolation of the transcribed counterpart in a cDNA clone and its further localization. The cDNA clone, from a lung fibroblast library, is assembled from three exons, including a 289 amino acid (AA) long open reading frame containing a recently described motif, the Kruppel-associated box, 42 AA long, in exon 2. By comparison with other reported members of the subfamily, the exon-intron boundaries also appear to be very well conserved. Further analysis allowed us to map this gene 1 Mb downstream from the HPRT gene in the published YAC contig that extends across Xq26. Two other motifs, 87 and 78% homologous to ZNF75 at the amino acid level, were identified by PCR on total human DNA, but map outside Xq24-qter.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalGenomics
Volume18
Issue number2
DOIs
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Genetics

Cite this

Villa, A., Zucchi, I., Pilia, G., Strina, D., Susani, L., Morali, F., Patrosso, C., Frattini, A., Lucchini, F., Repetto, M., Sacco, M. G., Zoppe, M., & Vezzoni, P. (1993). ZNF75: Isolation of a cDNA clone of the KRAB zinc finger gene subfamily mapped in YACs 1 Mb telomeric of HPRT. Genomics, 18(2), 223-229. https://doi.org/10.1006/geno.1993.1459