ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells

Maria Condello, Barbara De Berardis, Maria Grazia Ammendolia, Flavia Barone, Giancarlo Condello, Paolo Degan, Stefania Meschini

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Zinc Oxide (ZnO) nanoparticles are widely used both in the industry and in biomedical applications for their chemical and physical nanomaterial properties. It is therefore essential to go in depth into the cytotoxicity mechanisms and interactions between nanomaterials and cells. The aim of this work was to evaluate the dissolution of ZnO nanoparticles and their uptake, from a few minutes after treatments up to 24 h. ZnO nanoparticles routes of entry into the human colon carcinoma cells (LoVo) were followed at different times by a thorough ultrastructural investigation and semiquantitative analysis. The intracellular release of Zn2 + ions by Zinquin fluorescent dye, and phosphorylated histone H2AX (γ-H2AX) expression were evaluated. The genotoxic potential of ZnO nanoparticles was also investigated by determining the levels of 8-hydroxyl-2′-deoxyguanosine (8-oxodG). The experimental data show that ZnO nanoparticles entered LoVo cells by either passive diffusion or endocytosis or both, depending on the agglomeration state of the nanomaterial. ZnO nanoparticles coming into contact with acid pH of lysosomes altered organelles structure, resulting in the release of Zn2 + ions. The simultaneous presence of ZnO nanoparticles and Zn2 + ions in the LoVo cells determined the formation of reactive oxygen species at the mitochondrial and nuclear level, inducing severe DNA damage.

Original languageEnglish
Pages (from-to)169-179
Number of pages11
JournalToxicology in Vitro
Volume35
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Zinc Oxide
Nanoparticles
Colon
Cells
Carcinoma
Nanostructures
Nanostructured materials
Ions
Deoxyguanosine
Cytotoxicity
Endocytosis
Lysosomes
Fluorescent Dyes
Organelles
Hydroxyl Radical
Histones
DNA Damage
Reactive Oxygen Species
Industry
Dissolution

Keywords

  • Genotoxicity
  • Human colon carcinoma cells
  • Nanoparticles uptake
  • Oxidative stress
  • ZnO nanoparticles

ASJC Scopus subject areas

  • Toxicology

Cite this

Condello, M., De Berardis, B., Ammendolia, M. G., Barone, F., Condello, G., Degan, P., & Meschini, S. (2016). ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells. Toxicology in Vitro, 35, 169-179. https://doi.org/10.1016/j.tiv.2016.06.005

ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells. / Condello, Maria; De Berardis, Barbara; Ammendolia, Maria Grazia; Barone, Flavia; Condello, Giancarlo; Degan, Paolo; Meschini, Stefania.

In: Toxicology in Vitro, Vol. 35, 01.09.2016, p. 169-179.

Research output: Contribution to journalArticle

Condello, M, De Berardis, B, Ammendolia, MG, Barone, F, Condello, G, Degan, P & Meschini, S 2016, 'ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells', Toxicology in Vitro, vol. 35, pp. 169-179. https://doi.org/10.1016/j.tiv.2016.06.005
Condello, Maria ; De Berardis, Barbara ; Ammendolia, Maria Grazia ; Barone, Flavia ; Condello, Giancarlo ; Degan, Paolo ; Meschini, Stefania. / ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells. In: Toxicology in Vitro. 2016 ; Vol. 35. pp. 169-179.
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